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  • - Cindi Hein
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  • - Bob Morris

    Re: [RC] [RC] B.C.A.A. Complex - Lisa Redmond

    Pray tell us how you determine
    what is a "normal amount?"
    There are a couple of ways you can approach this.One is to design a diet
    that is (hopefully) completely deficient in that nutrient, and comparing the
    animals' performance on that control diet to animals receiving diets with
    varying levels of the nutrient.  Another possibility is to run a
    digestibility trial of sorts, where you again control the amounts of the
    nutrient, but you measure the appearance of the nutrient in the feces (or
    urine, where appropriate), which should be an indication that the animal's
    needs are being met and excess is being eliminated from the body.  This
    version makes the assumption that all amino acids that disappear are
    absorbed and utilized as amino acids.
    What has to be taken into consideration by the researcher is that fecal
    material always contains protein/amino acids that don't come from the feed
    but are endogenous.  Endogenous (for the folks who are sitting there saying
    "There she goes with the science lingo again") means that the protein (or
    lipid, or carbohydrate, or whatever) came from the body rather than the
    feed.  In the horse, this is primarily from the cells which slough off the
    intestinal wall (it renews itself every few days) and bacterial protein.
    You account for this by determining the amount that appears in the manure of
    the control group to establish a base line.  Then, anything above that
    amount is considered to be dietary in origin.
    These sort of trials are not exactly the most glamorous sort of research---I
    speak from experience!  However, it is pretty much the only way to establish
    nutritional requirements for something like protein or energy.  Done
    properly, they are time-consuming but effective methods of determining
    If you want to determine normal circulating amounts in the blood, or rate of
    absorption from the gut, you can use a nutrient that is labeled with a
    marker of some sort.  Frequently, this is accomplished by replacing a
    hydrogen atom with a tritium (radioisotope of hydrogen) on the molecule.
    The main thing that to be remembered is that for something to be considered
    a valid determination of a dietary requirement, there has to be ample data
    and statistical analyses to meet tradtitional scientific standards.  It also
    has to be repeatable....otherwise it stands a chance of ending up in the
    Journal of Irreproducible Results (a real publication...we used to pass them
    around the grad student office!).
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    Re: [RC] [RC] B.C.A.A. Complex, FASTGraphic
    Re: [RC] [RC] B.C.A.A. Complex, Heidi Smith
    Re: [RC] [RC] B.C.A.A. Complex, Jim Holland