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Authors SNJ Geelen, WL Jansen, MJH Geelen, MMS vanOldruitenborghOosterbaan, 
AC Beynen
Title   Lipid metabolism in equines fed a fat-rich diet
Full source International Journal for Vitamin and Nutrition Research, 2000, 
Vol 70, Iss 3, pp 148-152
Author keywords horse; dietary fat; lipoprotein lipase; adipose lipolysis; 
glycolytic flux; fatty acid oxidation

The hypothesis tested was that dietary fat, when compared with an 
isoenergetic amount of non-structural carbohydrates, stimulates lipolysis in 
adipose tissue and also stimulates the fatty-acid oxidative capacity in 
skeletal muscle from horses. Six adult horses were fed a high-fat, glucose or 
starch containing diet according to a 3 x 3 Latin square design with feeding 
periods of three weeks. The diets were formulated so that the intake of 
soybean oil versus either glucose or corn starch were the only variables. In 
accordance with previous work, whole plasma triacylglycerol (TAG) 
concentration decreased significantly by 58% following fat supplementation. 
This fat effect was accompanied by a 2476% increase in lipoprotein lipase 
(LPL) activity in post-heparin plasma. The dietary variables did neither 
significantly affect the basal in vitro lipolytic rate nor the lipolytic rate 
after adding noradrenaline. There was no significant diet effect on the 
activities of hexokinase and phosphofructokinase as indicators of glycolytic 
flux and citrate synthase and 3-hydroxy-acyl-CoA dehydrogenase as indicators 
of fatty-acid oxidative capacity. The concentrations of muscle glycogen and 
TAG were not affected by fat supplementation. It is concluded that our 
hypothesis is not supported by the present results.