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    [RC] FW: <fwd> Louse Flies Test Positive for West Nile Virus - Maryanne Stroud Gabbani


    WEST NILE VIRUS, HIPPOBOSCID FLIES - USA (PA)
    *******************************************
    A ProMED-mail post
    <http://www.promedmail.org>
    
    [1]
    Date: Tue 24 Sep 2002
    From: John P. Maher <jmaher@xxxxxxxxxx>
    
    Louse Flies (_Hippoboscidae_) Test Positive for West Nile Virus
    - ---------------------------------------------------------------
    A pool of Hippoboscid flies has tested positive for West Nile virus in
    Dauphin Co (Pennsylvania). These flies are also called louse flies and are
    ectoparasites on birds and mammals. We have no idea regarding the
    importance of these insects because no work has been done with respect to
    Hippoboscid vector competence for West Nile virus. If they are capable of
    vectoring West Nile virus, they could be very important enzootic vectors in
    the wild in roosting areas and among nest mates. Birds in captivity, such
    as those in zoos and bird rehab centers, could also be disproportionately
    affected.  The flies were collected from a symptomatic Great horned owl.
    
    - --
    John P. Maher
    <jmaher@xxxxxxxxxx>
    
    ******
    [2]
    Date: 25 Sep 2002
    From:  Hutchinson, Michael <mhutchinso@xxxxxxxxxxx>
    
    [In response to a query for additional information on the identification of
    West Nile virus in hippoboscidae, Michael Hutchinson was kind enough to
    send the following. - Mod.MPP]
    
    Thank you for your question about the positive louse flies.  We have sent 3
    pools of Hippoboscidae for West Nile virus testing.  The first pool of 5-6
    specimens was taken from a Red Tail (RT) hawk that tested positive for WN -
    that pool tested negative.  The second pool contained 5 specimens that were
    collected from a symptomatic Great Horned (GH) owl.  That is the pool that
    tested positive through PCR as well as through virus isolation, indicated
    the presence of live virus.  This is not surprising in that the specimens
    were visibly blooded.  The results from a 3rd pool of non-blooded specimens
    are still pending.  Future submissions of Hippoboscids from symptomatic
    birds will be held in the lab for 7-10 days to try to ensure complete
    digestion of the blood meal.  Positive results from non-blooded insects
    will give us a better idea if the virus can survive within the louse
    flies.  We will still be in need of vector competency studies to nail down
    the importance, or lack thereof, of this group.  As for the question about
    the identity if the flies, they are in the genus Icosta and I'm waiting on
    literature to provide a species level identification.  There are about 8
    species in North America and they are bird biters.
    
    Michael L. Hutchinson
    Biologist - West Nile Virus Program
    PA Department of Environmental Protection
    <mhutchinso@xxxxxxxxxxx>
    
    ------------------------------
    
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